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Project TitleAqueous Protein Extraction Process
Track CodeP27102
Short Description

AAFC has developed a pilot scale process to extract cruciferin‐ and napin‐rich co‐products from canola meal. This process is simpler and cheaper than the sole commercial alternative used by industry leaders in the canola protein sector. The AAFC process removes an allergen which has prevented these first canola proteins from receiving full regulatory approval as GRAS (generally regarded as safe) for use in food – a much higher value application than animal feed for which canola meal is typically destined.


The AAFC process largely separates the two major storage protein groups in canola: the 2 S napins and 12 S cruciferins. The process yields three protein co‐products (~30 percent dry basis) leaving a low‐protein residue of soluble sugars and seed coat (70 percent dry basis).
 5%: napin‐rich isolate
 5%: napin/cruciferin mixture
 20%: cruciferin‐rich isolate
 30%: total protein isolates
The process separates high‐purity napin and cruciferin by exploiting their divergent solubilities under different pH conditions, using unit operations commonplace in the food ingredients industry.
The addition of NHSO3 at low concentration prevents the reactions that result in dark‐coloured protein isolates which lower their value in food applications.
The salt added to maximize protein solubility is removed by ultrafiltration of the aqueous napin and cruciferin solutions to yield the two protein isolates. The optimum conditions for the entire separation process have been established to maximize product purity and yield.

Contact: Nancy Tyler, Commercialization Officer
Agriculture and Agri-Food Canada
107 Science Place
Saskatoon, Saskatchewan S7N 0X2 Canada

Tagsprotein extraction
Posted DateMar 25, 2015 2:31 PM


File Name Description
Business Opportunity Document None Download